Read the user manual operating the pikoreal realtime pcr. Thermo scientific piko thermal cycler marshall scientific. Applied biosystems proflex pcr system thermo fisher. We have not only shrunk the size of the plate, but dramatically reduced the thickness of the well walls utilizing our patented ultrathin wall utw technology. Applied biosystems 2720 thermal cycler user manual. Alpha cycler with blocks mains cables uk, eu, china and us usb stick containing operators manual and default protocols 2x 15a fuse which must be changed in certain territories and power supplies, see below. This thermal cycler uses neutral fusing, which means that live power could still be exposed inside the instrument even when the fuse is blown or. Thermo scientific tm piko thermal cyclers deliver top performance with twice the speed and only half the size of regular thermal cyclers. Multigene mini thermal cycler tc02024 tc02024230v tc05018 tc05018230v operation manual ver. The multigene optimax thermal cycler is a p owerful, reliable and affordable thermal cycler.
For some realtime instruments calibration dye may not be needed. In 1987 the first commercial thermal cycler, the tc1 dna thermal cycler. Refer to publication 1760gr001, pico controller getting results manual for a basic overview. Back that up with a robust optical system that acquires all channels simultaneously and running multiplexed assays has never been quicker. Intended use the pikoreal realtime pcr system is a thermal cycler with a fluorescence measuring system available in 24well and 96well formats.
The thermal cycler also known as a thermocycler, pcr machine or dna amplifier is a laboratory apparatus most commonly used to amplify segments of dna via the polymerase chain reaction pcr. Biorad icycler thermal cycler manual attachments biorad icycler thermal cycler manual 598 kb. Multiprobe tvk user guide for veriti thermal cycler, 16 veriti thermal cycler before you run 96well instrument tests physical injury hazard. Applied biosystems stepone and steponeplus realtime pcr systems reagent guide vii preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems stepone and steponeplus realtime pcr systems stepone and steponeplus systems. Functions which are available only for specific variants are indicated by a note such as this function is only available for gradient enabled thermal cyclers or this function is only available for thermal cyclers featuring quick block exchange. The piko 24well thermal cycler model piko 24 allows you to complete a pcr protocol in less than 15 minutes with the piko thermal cycler, which meets the highest criteria in thermal performance at twice the speed and half the size of other thermal cyclers. Thermal cyclers the aeris thermal cycler offers five interchangeable blocks designed to meet critical requirements for different applications. The driftcon software is also used to analyse the measurement data and compare this with the instrument market specifications. North america description mastercycler pro 1 6321 000. Thermo scientific pikoreal user manual pdf download.
All crossreferences refer to the thermo scientific piko thermal cycler user manual, rev 1. Thermo fisher scientific thermo scientific pikoreal software 2. The applied biosystems 2720 thermal cycler user manual describes how to use the applied biosystems 2720 thermal cycler. About this user manual intended users about this user manual intended users the pikoreal realtime pcr system, as a standalone instrument or with thermo scientific pikoreal software, can be used in research laboratories by professional personnel. Applied biosystems abi 9903 veriti 384 well thermal cycler. Mastercycler mastercycler gradient operating manual. Applied biosystems veriti thermal cycler thermo fisher. Veriti thermal cycler user guide vii preface how to use this guide purpose of this guide the applied biosystems veriti user guide provides information about installing, using and maintaining the applied biosystems veriti thermal cycler. The thermo scientific 96well piko pcr plates, clear, are a family of pcr plates which have the footprint of a microscope slide.
The piko 96 thermal cycler can complete a pcr protocol in as little as 15 minutes enabled by fast ramp rates and quick settling times. It describes the procedures you use to install, wire, and troubleshoot pico. The peqstar 2x, with two independent high speed 48well blocks in one system, offers the optimal solution for maximum flexibility. To insure adequate cooling of the mycycler, place it on a clean, flat surface, and be sure that there is at least 4 inches 10 cm clearance around each side of the thermal cycler. This manual provides instruct ions on how to safely set up a nd operate the c t ouch thermal cycler a nd uses the writing convention s shown in t able 2. Ideally, nanostring recommends a thermal cycler with a heated lid that can adjust throughout the protocol. C thermal cycler manual v instrument safety warnings the following warning labels displa y on the instrument, and refer directly to the safe use of this c thermal cycler table 4. The veriflex temperature control technology inside makes it possible to run up to 6 different temperatures in the same protocol step, providing precise control over your pcr optimization. The applied biosystems proflex pcr system combines the reliability and performance youve come to expect from applied biosystems thermal cyclers with the flexible configuration and control features that fit how you work today.
Cycle, resolved the need for manual addition of fresh enzyme after each cycle by using liquid handlers and water baths 1. About this user manual safety instructions thermo fisher scientific thermo scientific piko real realtime pcr system user manual 5 the following symbols and markings appear in this user manual. Piko pcr plate, 96well, clear thermo fisher scientific. Safety and regulatory compliance this instrument has been tested and found to be in compliance with all applicable. Simpliamp thermal cycler installation and operation quick. Deleting a user account is a permanent change and cannot be undone. The contents of the delivery package depend on the variant of your mastercycler pro. Audience this guide is intended for novice and experienced veriti thermal cycler users who. Plug in the unit to provide power, there is no toggle onoff switch. Piko thermal cyclers are available with two different block configurations. The user can now log in with his or her pin at any time. Minicycler operations manual meet the ptc150 minicycler thank you for purchasing an mj research ptc150 minicycler.
Its purchase price includes the upfront fee component of a license under the patents on the polymerase chain reaction pcr process, which are owned by hoffmannla roche inc. Finnzymes instruments piko 96 well thermal cycler marshall. Thermo scientific piko thermal cycler 96well piko thermal. Instrument specifications instrument specifications. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Deleting a user account will delete the respective user node including all folders and programs it contains. Picoplex single cell wga kit user manual takara bio. Geneamp pcr system 9700 with 96well goldplated silver sample block. There are no user serviceable parts for this instrument. Miniamp and miniamp plus thermal cycler user guide pub. Page 2 the information in this guide is subject to change without notice. Therefore, engineers started to look into inventing an allinone instrument thermal cycler that would help automate the pcr process. The driftcon software is used to capture the temperature data from the driftcon hardware box and store it in a data file, along with identification parameters.
User friendly ergonomic design, small footprint to conserve. Place the t100 thermal cycler base on a flat, dry surface with. The alpha cycler thermal cyclers are standalone systems with flexible block options 96 or 384 well formats being available. Thermal cyclers are essential laboratory equipment for researchers who run polymerase chain reaction pcr for sequencing, cloning, genotyping, mutagenesis, and many other applications. Mic uses a patented magnetic induction technology to heat your samples and fan forced air for cooling. C touchtm thermal cycler manual iii safety and regulatory compliance the c touch thermal cycler heats and cools very quickly during operation. To open the user documentation on the veriti thermal cycler. For detailed instructions on using the thermal cycler, see the simpliamp thermal cycler user guide pub. Designed to deliver bridled power, it results in industryleading thermal uniformity, accuracy, ramp rates and settling times. The thermo scientifictm arktiktm thermal cycler is a reliable and flexible pcr instrument for everyday requirements in any laboratory. The peqstar x cycler is designed around a powerful yet easy to use software interface, and uses the same platform for both 96well and 384well formats. The user is advised to keep the original packaging in case the instrument ever needs to be returned for service or repair. To put into standby mode conserving power, hold down for 3 sec 4. Specification sheet simpliamp thermal cycler key features compact design large, color lcd touchscreen simpli.
The pcr chamber can hold up to 24 samples in easy to use sample strips. Available realtime rtpcr cycler and kinetic pcr machines. K series is available in a range of automated and manual models with the. S thermal cycler manual v reasonable protection against harmful interference when the equipment is operated in a commercial environment. The miniamp thermal cycler and the miniamp plus thermal cycler are endpoint thermal cyclers, specifically designed for the amplification of nucleic acids using the polymerase chain reaction pcr process. Takara pcr thermal cycler dicetm touch is more small pcr device and easy to use the big touch panel, that reconfigure takara pcr thermal cycler dicetm series as usual. A portable document format pdf version of this guide is available on the veriti thermal cycler documentation cd. Easytoread programming interface for quick and painless. Page 1 simpliamp thermal cycler user guide installation, use, and maintenance for use with. Only a logged in administrator may access the admin menu. We strongly recommend that you follow the safety specifications listed in this section and throughout this manual. Designed by a team of molecular biologists and engineers, the minicycler will meet your needs for an easytouse, reliable, and compact programmable thermal cycler.
Pcr instrumentation 09 10 px2 thermal cycler advanced personal thermal cycler capable of performing anything from simple to the most sophisticated pcr protocols accurate temperature control multiple interchangeable block options. Picofuge with strip tube adaptor stratagene or equivalent. Large, expensive thermal cyclers that take up an entire workspace are replaced with an. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. Interchangeable block formats allow you to maximize your throughput or run independent experiments concurrently. Multiblock capability with automatic block recognition software minimizes the need for manual settings. How to use the simpliamp thermal cycler operate the instrument using the touchscreen. Coleparmer, beacon road, stone, staffordshire, st15 osa, uk. Common features of biorads thermal cyclers include peltier effect technology for precise temperature control, and thermal gradient to easily optimize pcr. Purpose of this manual this manual is a reference guide for pico controllers. Please read this manual carefully before using the thermal cycler. Morrison 42015 page 3 hot link to biorad mycycler user manual 45 pgs pdf 1.
View and download biorad mycycler instruction manual online. The finnzymes instruments piko 96 well thermal cycler is an ideal solution for both conventional and efficient pcr applications. All these data and results are summarized in a driftcon report. It is designed to help you quickly learn to use the veriti thermal cycler. Pcr express thermal cycler user instruction manual head office thermo hybaid action court ashford road ashford middlesex tw15 1xb telephone.
Thermal cyclers may also be used in laboratories to facilitate other temperaturesensitive reactions, including restriction enzyme digestion or rapid. Piko thermal cycler 96well system from thermo fisher. This cfx96 or cfx384 detection module, when combined with a c thermal cycler. T100 thermal cycler power cord support ring for individual tubes instruction manual remove all packaging materials and store them for future use. The administrator has access to all user and admin functions. Connect the free end of the ribbon cable from the multiprobe. Applied biosystems simpliamp thermal cycler user manual pdf. This means fast qpcr results through rapid heating and cooling with 35 cycles possible in under 25 minutes. Applied biosystems abi applied biosystems pcr applied biosystems 2720 thermal cycler tags. Piko thermal cycler 24well system from thermo fisher. The aeris thermal cycler offers five interchangeable blocks designed to meet critical requirements for different applications. If any item is missing or damaged, contact your local biorad office. This user manual covers the use of pikoreal software in conjunction.
No right is conveyed no right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits or methods such as 5 nuclease methods. Power supply connector drawer open feet air intake air exhaust. Authorized thermal cycler this instrument, serial no. Do not slide any paper or other material under the system as this may hamper the airflow or be sucked into the system. Thermo fisher scientific thermo scientific piko thermal cycler user manual 35 chapter 7 technical specifications thermo fisher scientific reserves the right to change any specifications without prior notice as part of our continuous product development program table 71. This not only allows for pcr protocols in as little as 10 minutes, but also assures consistent results from welltowell and instrumenttoinstrument. The user manual provides information about performing runs and diagnostic tests as well as maintenance and troubleshooting. C touchtm thermal cycler manual once a user is logged in as the administrator, the user and admin menus are visible. In the navigation tree, a new user node is displayed with this user name. Text conventions this guide uses the following conventions. Whats included user manual and power cord description 3 prime base thermal cycler, 24 x 0.
Thermal cyclers for pcr life science research biorad. Overview thank you for purchasing takara pcr thermal cycler dicetm touch. Summary of contents for applied biosystems simpliamp thermal cycler page 1 simpliamp thermal cycler user guide installation, use, and maintenance for use with. The pikoreal realtime pcr system is designed to fit into your lab, even when. At the heart of the piko thermal cycler lies our unique heat pump. Use care when working around the heated cover and sample blocks to avoid being burned. The geneamp pcr system 9700 thermal cycler is covered by us patent claims and corresponding claims in their nonus counterparts. If the equipment is not used in the manner described in this manual the protection provided by the equipment might be impaired. Invitrogen and applied biosystems pcr reagents catalog number a24811 publication number man0009889 revision c. Documents document view page applied biosystems 2720. Thinwalled pcr tubes are highly recommended for use with stratagene robocycler thermal cyclers. The peqstar thermal cycler family combines high quality engineering with a comprehensive range of block formats. The piko thermal cycler has an easy to use and very intuitively designed user interface.
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